PSILOCYBIN QTEST

Psilocybin-QTest Instruction Manual

For a quick overview of how to perform a test see the Quick Reference Guide (slider above).

Please find the most recent version of the detailed instructions below. You always get a paper version of these instructions with your test kit.

Frequently Asked Questions about the Psilocybin-QTest

Do I need anything else to perform the test?

Everything you need is included in the box.

To prepare your sample, you only need the following, which you probably already have at home:

• A grinder or scissor for grinding & homogenizing the sample
• A milligram scale to weigh 150 mg for the test
• A small funnel or a creased piece of paper to pour the mushroom material into the extraction vial
• A pot to boil water and a pair of kitchen tongs to incubate the color reaction and safely remove the detection vial for evaluation

How much material do I need for the test?

You only need 150 mg (0.15 g) of your sample to perform the test. It is best to grind down a larger batch, mix it well, and then test only 150 mg of this homogenized batch. The result will tell you the potency for the remaining material.

Does the QTest also test for Psilocin?

The Psilo-QTest quantifies the total alkaloid content (psilocybin + psilocin) and reports the summed values as a total psilocybin concentration.

Does the Psilo-QTest also work for extracts? If I want to know the potency of the extract and not the mushrooms it was made from, do I put 150 mg of the extract?

What does quantitative test mean and how does the QTest differ from “classic” reagent tests?

Our QTests are more powerful than classic reagent tests. While reagent tests detect the presence of an active ingredient in a substance, our QTests quantify it. This means they determine the concentration of active ingredients in various psychoactive substances, such as magic mushrooms or magic truffles.

What is the shelf life of the QTest, and how should I store it?

Store the reagents in the refrigerator (2°C - 8°C) and out of direct sunlight for a shelf life of at least 18 months. The extraction liquid may turn slightly yellow over time. That’s okay. It is still usable.

How can I prevent the filter from clogging?

We recommend ensuring that floating fungi are not sucked into the system and positioning the sterile filter below the floating fungus layer before applying vacuum. If the process fails initially, cleaning the filter very carefully and reapplying the syringe can help. The test can still function if fungal components are transferred, but this may complicate color evaluation. Link to our video.

Do solvents interfere with the measurement?

The tests work well with, for example, rotated or evaporated ("dry") extracts, but also some solvents like ethanol work if the concentration of alkaloids is not too low. This means that if too many foreign solvents are added to your extraction, it will disturb your extraction system. The best way would be to evaporate the ethanol or any other solvent from a defined amount, note the dry weight, and measure it with the test.

Haven't found the answer you’re looking for?

Click here to find more FAQs or contact us via info@miraculix-lab.de

Verification & Testimonials of the PSILOCYBIN QTEST

Compliant with established pharmaceutical testing criteria - Pharmaceutical Validation ICH Q2(R1)

Our QTests were developed by our scientist, Dr. Felix Blei, in the laboratories of the University of Jena in Germany. It is a voluntarily validated and standardized test procedure based on the pharmaceutical method validation guideline ICH Q2(R1), which we have extended to the QTests. This guideline describes the requirements of the analytical method for active ingredients that are subsequently to be used in human medicine and guarantees a safe dosage of these active ingredients, for example in pharmacies.

The PSILO-QTest was developed at the Friedrich Schiller University Jena at the Institute of Pharmacy, Chair of Pharmaceutical Microbiology, under grant number 03EGSTH1189, supported by the German Federal Ministry of Economics and Climate Protection and the European Social Fund, in the course of the third-party funded project "Production of quantitative test systems of psychotropic agents" in the period from 2019 to 2021 under the project management of Dr. Felix Blei. The necessary permission for the handling and acquisition of the narcotics psilocybin/psilocin according to § 3 of the German Narcotics Act was granted for the Friedrich Schiller University Jena under the current BtM number 463 23 75 as well as the previous one for the premises of the Pharmaceutical Microbiology in Jena. The pure substances required for the project were purchased from LIPOMED GmbH and LGC Standards Ltd.

Interested in learning more? Please take a look at our short validation of the Psilocybin-QTest

Validation by authorities - State-regulated drug checking

The QTest where officially validated & approved for the federal drug-checking program ALIVE in the federal state of Thuringa (Germany). They have already been used on-site for 3 years and form the backbone of the mobile analysis methods. An evaluation of mobile drug checking is currently being prepared for publication by the Berlin Charité.

Confirmation from others

The functionality and the precision of our QTests have already been tested & validated by a lot of customers and third parties:

Psilocybe Expert & Shroomery Community founder Alan Rockefeller is a convinced user of our QTests, straight from the beginning. He uses the Psilo-QTests to quantify the psilocybin in rare species like in the YT Series "crime pays but botany doesn't"

Doma aka Magic Myco, has extensively analyzed our tests in parallel to his HPLC analysis and pure standards, as well as already evaluating our tests with spectrophotometric methods:

Psychedelic Passage compared the results of our rapid tests with laboratory tests from Energy Control International (a long-standing drug-checking program in Spain) for identical samples and come to convincing results

• Psilocybin Q-Test Result: According to our at-home test, there is approximately 1.1% psilocybin and psilocin combined in a 1-gram sample of our mushrooms
• Psilocybin HPLC Test Result: The lab analysis revealed a psilocybin content of 1.08% psilocybin and psilocin combined

"The results from the two tests are very close. The slight difference between the at-home test prediction and the lab test result is negligible. Considering that both methods can fluctuate within 10% in accuracy, the minor variation is well within the acceptable range of variation. This indicates that Miraculix QTests are reliable tools for assessing the potency of your psilocybin mushrooms."

Important Disclaimer

This test is not suitable for identifying Psilocybin/Psilocin or psilocybin-containing mushrooms. For example: When picking wild mushrooms, there is a risk of confusing or mixing them with the toxic Galerina Maginata, which grows in similar habitats. The QTest does not test for fungal toxins, so it cannot be used for mushroom identification. However for test psilocybin-containing mushrooms, the QTest quantifies the amount of the active substance present.

Psilocybin is not uniformly distributed within mushrooms! Even mushrooms from the same batch can vary significantly in their psilocybin content. To ensure accuracy, it is essential to homogenize the mushroom material thoroughly by using a grinder, sharp knife, or cutting board before conducting the test. The test result will indicate the percentage of psilocybin per gram in the homogenized material.  The total measurement will be presented in terms of psilocybin, encompassing both psilocybin and psilocin.

IMPORTANT:

Testing a sample with our test kits should not be considered a guarantee of safety. Essential risks are associated with the consumption of any substance. Various factors can influence testing outcomes and reactions. Miraculix does not take responsibility for the interpretation of test result. Sample responses can differ due to lighting variations and specific substance blends being tested. The use or misuse of this information is not the responsibility of miraculix.