Frequently Asked Questions about the Psilocybin-QTest
Do I need anything else to perform the test?
Everything you need is included in the box.
To prepare your sample, you only need the following, which you probably already have at home:
- A grinder or scissor for grinding & homogenizing the sample
- A milligram scale to weigh 150 mg for the test
- A small funnel or a creased piece of paper to pour the mushroom material into the extraction vial
- A pot to boil water and a pair of kitchen tongs to incubate the color reaction and safely remove the detection vial for evaluation
How much material do I need for the test?
You only need 150 mg (0.15 g) of your sample to perform the test. It is best to grind down a larger batch, mix it well, and then test only 150 mg of this homogenized batch. The result will tell you the potency for the remaining material.
Does the QTest also test for Psilocin?
The Psilo-QTest quantifies the total alkaloid content (psilocybin + psilocin) and reports the summed values as a total psilocybin concentration.
In addition to the application on dried fungal material, the rapid test shows equally reliable results for most solvent-free extracts.
Extracts are usually more concentrated. To ensure that the color result of the test still fits the scale on the evaluation card, you have to reduce the amount of starting material accordingly. Before testing homogenize your substance. Typically, methanol extracts are triple-concentrated, so if you have a crude extract, we recommend dividing the amount you use for the test by the factor three (50 mg). If the extract is considerably more concentrated, reduce the initial quantity accordingly. The extract must be as solvent-free as possible, as this can interfere with the extraction solution. Remember that you have to multiply the result of the color scale in the end with the used factor to get the actual result for the remaining sample. For example, weigh 50 mg (0.05 g) of material for a 3X extract. Weigh 15 mg (0.015 g) of material for a 10X extract.
What does quantitative test mean and how does the QTest differ from “classic” reagent tests?
Our QTests are more powerful than classic reagent tests. While reagent tests detect the presence of an active ingredient in a substance, our QTests quantify it. This means they determine the concentration of active ingredients in various psychoactive substances, such as magic mushrooms or magic truffles.
What is the shelf life of the QTest, and how should I store it?
Store the reagents in the refrigerator (2°C – 8°C) and out of direct sunlight for a shelf life of at least 18 months. The extraction liquid may turn slightly yellow over time. That’s okay. It is still usable.
How can I prevent the filter from clogging?
We recommend ensuring that floating fungi are not sucked into the system and positioning the sterile filter below the floating fungus layer before applying vacuum. If the process fails initially, cleaning the filter very carefully and reapplying the syringe can help. The test can still function if fungal components are transferred, but this may complicate color evaluation. Link to our video.
Do solvents interfere with the measurement?
The tests work well with, for example, rotated or evaporated (“dry”) extracts, but also some solvents like ethanol work if the concentration of alkaloids is not too low. This means that if too many foreign solvents are added to your extraction, it will disturb your extraction system. The best way would be to evaporate the ethanol or any other solvent from a defined amount, note the dry weight, and measure it with the test.
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Without performing this test, it was impossible to determine the actual psilocybin dose I had ingested. Subjective effects alone do not allow for any reliable estimation of psilocin content, and this uncertainty was further compounded by the use of different strains from different harvests
Worked as expected
Great product! Well packaged and with detailed instructions, 5/5 stars!
These test kits are a lifeline to those of us who want to use psychedelics responsibly and minimize potential harm. I grow my own psychedelic mushrooms as it’s legal where I live. But mushrooms can vary significantly in their potency, and I feel it’s essential to test every batch before I use them. This is the only test kit I’ve found that is quantitative, accurate and reliable. I’ve been using these kits for years and I highly recommend them to anyone who grows shrooms, and ESPECIALLY to anyone who buys them from someone else. The difference between a psylocibin experience that is healing and spiritual, and one that sends you to the hospital is knowing the dose you’re taking.
I have been using the Psilocybin Qtest for a number of years now and find them very important with the wide range of potency you might find based on strain, drying and storage method. The tests are relatively easy to use and reliable!